Computational and Analytical Modeling of Cationic Lipid-DNA Complexes
نویسندگان
چکیده
منابع مشابه
Computational and analytical modeling of cationic lipid-DNA complexes.
We present a theoretical study of the physical properties of cationic lipid-DNA (CL-DNA) complexes--a promising synthetically based nonviral carrier of DNA for gene therapy. The study is based on a coarse-grained molecular model, which is used in Monte Carlo simulations of mesoscopically large systems over timescales long enough to address experimental reality. In the present work, we focus on ...
متن کاملModeling of cationic lipid-DNA complexes.
Cationic lipid-DNA complexes, often referred to as lipoplexes, are formed spontaneously in aqueous solutions upon mixing DNA and liposomes composed of cationic and nonionic lipids. Understanding the mechanisms underlying lipoplex formation, structure and phase behavior is crucial for their further development and design as non-viral transfection vectors in gene therapy. From a physical point of...
متن کاملComputational modeling of DNA-cationic lipid complexation.
Developments in gene therapy have led to a need for new and more efficient transfection vectors. So far, the use of viruses has received major attention. During the recent years, however, cationic liposomes have emerged as a potential rival for viruses and have several advantages, e.g. nonimmunogenity, low toxicity and a capability to carry larger amounts of DNA. Yet, the properties of cationic...
متن کاملThe structure of DNA within cationic lipid/DNA complexes.
The structure of DNA within CLDCs used for gene delivery is controversial. Previous studies using CD have been interpreted to indicate that the DNA is converted from normal B to C form in complexes. This investigation reexamines this interpretation using CD of model complexes, FTIR as well as Raman spectroscopy and molecular dynamics simulations to address this issue. CD spectra of supercoiled ...
متن کاملThe phase behavior of cationic lipid-DNA complexes.
We present a theoretical analysis of the phase behavior of solutions containing DNA, cationic lipids, and nonionic (helper) lipids. Our model allows for five possible structures, treated as incompressible macroscopic phases: two lipid-DNA composite (lipoplex) phases, namely, the lamellar (L(alpha)(C)) and hexagonal (H(II)(C)) complexes; two binary (cationic/neutral) lipid phases, that is, the b...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2007
ISSN: 0006-3495
DOI: 10.1529/biophysj.106.096990